ABS reader ranges from 340-850 nm in wavelength and the ABS Plus reader ranges from 190-1000 nm, which is equivalent to having 510 or 810 free filters, respectively. For FXII, at various times, 90 μL of reaction was mixed with SBTI (500nM) and S-2302 (0. SpectraMax Microplate Readers. When you do a read at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. Laser Scanning Confocal Microscopy The fluorescent signals were obtained using a Zeiss LSM 510 laser scanning confocal microscope. These isotypes cause antigen cross-linking by multimeric binding and self. Data were expressed as mean percentage of viable cells. Afterwards, absorbance at 595 nm was measured with spectrophotometer (SpectraMax 340, Molecular Devices, USA). Amino acid analysis was performed on a Pico-Tag amino acid analyzer (Wat er System) as. 5 ng/ml; estradiol: 10 pg/ml. Immunofluorescence and Aggregation Analysis. Optical density was measured at 570 nm using a microplate reader (SpectraMAX 340, Molecular Devices Co. • TRF-based with 340/80 nm EX and 616/10 nm EM 0200-7027: EX range: 340/80 nm EM range: 616/10 nm: High fg levels of Streptavidin: High. Absorbance was then measured at 540 nm (isosbestic point of cytochrome c) and 550 nm (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). Features showcased by this model include temperature control, a robotics-compatible interface and Data Analysis Software. The mixture was centrifuged for 10 min at 10,000 rpm, and BSA concentration in the supernatant was determined using bicinchoninic acid (BCA) assay. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. SPECTRAmax® 190. 1 in stock! Molecular Devices SpectraMax M2 Multilabel Microplate Reader. SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. 2. Liquid handling for random screening was carried out with a Biomek FX from Beckman Coulter (Fullerton, CA, USA) and HYTRA. 340-850 nm: Wavelength Selection: Monochromator, turnable in 1 nm increments:. All strains were tested three times and the results averaged. Background: Ketamine can act as a multifunctional neuroprotective agent by inhibiting oxidative stress, cellular dysfunction, and apoptosis. Warranty. 6. ATI Note: #1: The N-34-MLA is a replacement assembly which replaces an OEM lamp. Operators can conveniently select up to 6 wavelengths at a time for absorbance detection in the visible wavelength range 340 nm - 850 nm. Labsystems Luminoskan RT Microplate Reader. 340 to 740 nm (linear variable dichroic) 220 to 1000 nm for abs (spectrometer) Plate Formats: 6 to 384-well plates, LVis Plate with sixteen 2 µL microspots. The control panel consists of a 2-x-20-character LCD and eleven pressure-sensitive membrane keys that can be used to control some functions of the instrument. Six wells were assayed for each set of conditions and stadard deviations. Intra- and inter-assay coefficients of variation were lower than 8. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. Wells were washed three times. Values were determined according to a standard curve (log/log curve-fit). The area under the curve (AUC) for the comparison of the growth rates was determined using. The metals were preincubated with A for 5 min when added before heme. 5 stars - 1602 reviewsThis assay typically develops in 1. 4. #ABS PLUS) SpectraMax® Plus 384 Microplate Reader (Molecular Devices cat. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. automated SpectraMAX 340 (Molecular Devices, Sunnyvale, CA, USA) microtiter plate reader. Number of flashes. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. (7. Details. AoSMCs were seeded into 6-well culture plates. Manager: Yinghua Chen, PhD Contact Email: yinghua. Please see photos. 2. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. The reaction was stopped with 1 M hydrochloric acid after 15 min. VersaMax reads 96-well and strip-well plates. SpectraMax Multi-Mode Microplate Readers. SpectraMax® M2/M2e user guide a multi-detection microplate reader with two-mode cuvette port SpectraMax M2e user guide cover 1 1 4/21/06 9:53:28 AM. Molecular Devices Spectramax 340 Absorbance Plate Reader (1997) Molecular Devices SpectraMax i3x Multi-Mode Microplate Reader (2016) Vernier UV-VIS Spectrophotometer (5) Vernier VIS Spectrophotometer (30) Infrared Spectroscopy. This is a used Molecular Devices SpectraMax 340PC Microplate Spectrophotometer. The chamber contains. Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. Values were determined according to a standard curve (log/log curve-fit). 340 nm / 70 nm. According to LDH assay, a 60 μL of reaction mixture containing catalyst (diaphorase/NAD+), dye solution of iodotetrazolium chloride and sodium lactate was. Not all manufacturers' microplates are the same with regard to design, materials, orSpectraMaxABS Plus,SpectraMaxM2,SpectraMaxM2e,SpectraMax M3,SpectraMaxM4,SpectraMaxM5,SpectraMaxM5e,SpectraMaxPlus 384 0200-7200 Multi-Mode ValidationPlate. 0200-7027. The assays contained 0. 4. Values are expressed as the mean s ± SD of 3 separate experiments, each performed in triplicate. application or budget. 190–850 nm <± 1. Percent Transmission Absorbance Competitor 0. Details. The plates were incubated for 4 additional hours at 37°C. 2 M DEEMM were mixed in a microtube,. PGD2 concentrations in samples from treated cell cultures were determined using the linear range of a standard curve and are presented as percentages of the PGD2 levels measured in the solvent controls. According to LDH assay, a 60 μL of reaction mixture containing catalyst (diaphorase/NAD+), dye solution of iodotetrazolium chloride and sodium lactate was. The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurements in the visible range. 30. 306 plate air cal fail,low light. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. The. Unit powers. 304 signal level saturation. A. The SPECTRAmax 340PC can mea-The extracted stain was then transferred to a 96-well plate, and the absorbance at 562 nm was measured using a SpectraMax 340 plate reader/spectrophotometer(Molecular Devices Corp. One plate carrier adapter is provided with the instrument. When you do a read at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. 2. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. The SpectraMax 384 Plus lets you get absorbance measurements from 190 to 1000 nm quickly for samples in test tubes, cuvettes, and 96- or 384-well microplates. Filipin StainingSpectraMax® 340PC384 microplate reader provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro data analysis software. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-ModeOpening the Drawer Manually on page The SpectraMax ABS can accommodate standard 96-well plates. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. Although it has been proven to be effective in various neurologic disorders, the mechanism of the treatment of traumatic brain injury (TBI) is not fully understood. 5), L-ascorbic acid (25. SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. addresses the needs of life science researchers who quantify very small amounts of DNA, RNA, oligos, and proteins. The activity was measured in a SpectraMAX 340 plate reader at 37°C and 410 nmol/l for 120 sec. Analytical Data Regression and Acceptance Limits The conversion of the optical density units for the test samples, VSs, and QCs to concentrations was performed using the Watson LIMS software (version. Donor: 620 nm. Or scan up to 384 wells at any time to determine the best assay conditions. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1. Manual: SoftMaxPro User Guide Manufacturer: Molecular Devices Location: SOM-E536. 5. 4- to 5-weeks old female BALB/c mice were purchased from Charles River Laboratories (QC, Canada). , Sunnyvale, CA). Fibrin clots were formed as described above with some modifications 46. A. , Sunnyvale, CA, USA) at 595 nm. below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. The levels of rheumatoid factor (RF) antibodies were measured at the Clinical Immunology Laboratory of the Sahlgrenska University Hospital or Leiden Clinical Immunology Laboratory. IC 50 values were determined by mathematical curve-fitting (SoftMaxPro Molecular Devices) and were defined as the drug concentration leading to 50% inhibition of net cell mass. 5 mL sample or standard solution was well mixed with 0. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. Microplate Readers. About GMI provides new and GMI Certified Pre-Owned scientific instrumentation and service to research universities, environmental labs, forensic investigators, biotechnology and pharmaceutical companies, hospitals, clinics and analytical laboratories, in the United. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. ), which was proportional to the number of damaged cells. 315 can't find zero order. FlexStation® 3 Multi-Mode Microplate Reader. 6 (h) x 22. 1. 314 low lamp level warning. ELISAs were read with a Spectramax 340 from Molecular Devices (Sunnyvale, CA, USA). Acceptor filter: 665 nm. The assay was carried out in a 96-well plate in a final volume of 100 µl containing 50 mmol/l Tris-HCl, pH 7. , Sunnyvale, CA) after incubation of solution mixture at 37 °C for 2 h 30 min in the dark. The plates were incubated for 4 additional hours at 37°C. 312 gain calibration failed. Figure 1. 8. SpectraMax Multi-Mode Microplate Readers. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. Subsequently, the oxidative burst was initiated by the addition of PMA (final concentration 100 nM) and the cells incubated for 120 min at 37°C. Measurements of OD at 540 nm were then performed in a microtiter plate reader (SpectraMax 340 microplate reader; Molecular Devices Corp. PerkinElmer offers a variety of Microplates For Absorbance and Colorimetric Assays. Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax. 5. Near bottom. 2. Apoptosis was also measured by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) assay using the TACS Blue label kit (4811-30-K, R&D Systems). SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. #W50-100) SpectraMax® ABS Plus Microplate Reader (Molecular Devices cat. Wavelength Range. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that. Proteolytic activity was determined using the synthetic chromogenic substrate Nα-benzoyl-DL-arginine p-nitroanilide (DL-BApNA), in 96-well plates, according to Ponce-Soto et al. , Sunnyvale, CA). Absorbance plate reader, temperature control, robotics-compatible, 384 well plates, 340-850 nm. Molecular Devices ThermoMax is a high-efficiency, temperature-regulated, 96-well microplate reader. Call 1-800-745-2710. Related Products: SpectraMax 340 Absorbance Microplate Reader, Molecular Devices SpectraMax 384 Plus UV/Vis Microplate Reader, Molecular DevicesGETTING THE MOST FROM YOUR MOLECULAR DEVICES MICROPLATE SYSTEMS applications guide to microplate systems applications guide to microplate systems450 nm using a SpectraMax 340 microplate reader (Molecular Devices, Sunnyvale, CA, USA). 0. Not. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. The VersaMax and SpectraMax 190 read 96-well microplates. After washing, the dye was eluted with 3% HCl, and the absorbance measured with a SpectraMax 340 microtiter plates reader at 665 nm. 1% Chaps, 10 m M dithiothreitol, and 10% sucrose. 0 1. The top- and bottom-reading Gemini EM Microplate Reader with dual monochromators lets users determine the optimal excitation and emission settings for creative new fluorescence. Integration delay. Photometric Performance SpectraMax 340PC384 Item Description Wavelength range 340 nm to 850 nm Wavelength selection Monochromator tunable in 1 nm increments Wavelength bandwidth. 0. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Although it has been proven to be effective in various neurologic disorders, the mechanism of the treatment of traumatic brain injury (TBI) is not fully understood. Methods: We investigated growth inhibition by varying concentrations of AMB in seven isolates of C. The activity was measured in a SpectraMAX 340 plate reader at 37°C and 410 nmol/l for 120 sec. The plate was read on a SpectraMax 340 PC plate reader (Molecular Devices, Sunnyvale, CA) at 450 nm, with reference at 650 nm. 00. The first time you replace an OEM lamp you must use the N-34-MLA. Near bottom. Cell viability was determined using the blue formazan assay in which colorless 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) is metabolized to a blue product by mitochondrial dehydrogenases. Upgradeable modules are also available including western blot, cell imaging, and fast. StakMax. View the Molecular Devices website. 1 mL of culture media with 10% FBS. Created Date: 20051213090753Zmeasured using a SpectraMax 340 multiwell plate. 5 mL of 1 M acetate buffer (pH 4. 2. The SpectraMax ABS readers use a grating monochromator to select the exact wavelength needed for every assay. The relative LDH release was determined as the percentage ratio of the optical densities in the media (containing CNMs at each. Glutathione reductase (GR) activity was measured, according to Smith et al. The drawer will open and it may close, if is doesn't, close it using the DRAWER button found on the Spectramax control panel (pictured in purple on. #ABS PLUS) Methods. Total Cell Count in BALF Cells collected from the lavage fluid were suspended in 1 mL of F-12 media supplemented with 10% FBS. Polystyrene plates (384-well) were precoated overnight at 4°C with 2 μg/ml of murine, human, or chimeric RANKL. NO release assayThe SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. 1x10 5 transduced, GFP + SK-NEP-1. Read up to 6 wavelengths in endpoint or kinetic mode for 96- and 384-well plates, and perform spectral scans to determine. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. The absorbance was measured at 405 nm on a SpectraMax 340 (Molecular Devices). Microplate Readers. Page 1 ® SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0049 Rev. Microplate Reader Shelf. Vessel Sprout Formation Assay. The concentration of Alizarin Red S staining in the samples was determined by comparing the absorbance values with those. Molecular Devices SpectraMax M5e Microplate Reader $26,500. Cell viability wasDescription Microplates The SpectraMax Multi-Mode Microplate Reader can accommodate SBS- standard 6-well to 384-well microplates and strip wells. N-34-LB. Tunable for filterless flexibility. CSF-1, IL-34 and calprotectin levels were analysed by ELISA according to instructions provided by the manufacturer (R&D Systems, Minneapolis, Minnesota, USA). The Molecular Devices SpectraMax 340PC384 Microplate Reader can run both standard spectrophotometer and microplate reader applications on the same instrument. This was followed by an additional incubation at 37 °C for 30–60 min. Microplate Spectrophotometers. 0, 20 mmol L –1cal-cium chloride). The antibodies originally defining this molecule by inducing apoptosis, anti-Fas and anti-APO-1 , were of the IgM or IgG3 subtype, respectively. Unit has several areas of damaged plastic. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. 190–850 nm <± 1. , Sunnyvale CA). When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. the SpectraMax iD3 is a fantastic plate reader for a wide range of assays. SpectraMax Multi-Mode Microplate Readers. used. The reaction was then stopped by adding 50 µL of 1 M sulfuric acid to each well. The SpectraMax ABS Plus, can accommodate standard 96-well plates and 384-well plates. 303 unable to cal dark current. Not all manufacturers' microplates are the same with regard to design, materials, or76358-624. With a wide range of on-board features and capabilities, thermoax is ideally suited for use in modern laboratories involved in many applications. HTRF-compatible SpectraMax Multi-Mode Readers. Excitation 1 340 nm Emission 1 520 nm Cut off for emission 1 495 nm Excitation 2 340 nm Emission 2 620 nm Cut off for emission 2 570 nm Sensitivity Readings 50 to 100 PMT Optimal Column Wavelenght Column Priority . Absorbance was read at 405 nm on a microplate reader (SPECTRAmax 340 PC). The SpectraMax® i Series Multi-Mode Mode Microplate Readers offer the ideal solution for any . TDP-43 stress granular cells were treated with 70% ice-cold. control or celatrol-treated cells. In order to detect the Fc-tagged fusion proteins, goat anti-human IgG (Fc Fragment specific; Jackson ImmunoResearch Laboratories Inc, West Grove, PA, USA; Code Number 109-006-098;. It measures the optical density (OD) of samples in both 96- and 384-well microplates at a selected wavelength for a single point in time (endpoint), over a specified period of time. GE Healthcare spectramax 340 pc plate reader Spectramax 340 Pc Plate Reader, supplied by GE Healthcare, used in various techniques. Incubate 30 min at room temperature, and then read microplates at 405 nm using SpectraMax 340 PC plate reader (Molecular Devices) (see Note 8). 1 mL of culture media with 10% FBS. Immunofluorescence and Aggregation Analysis. The absorbance at 540 nm was determined in a spectrophotometer (SPECTRAmax 340, Molecular Devices). SpectraMax Multi-Mode Microplate Readers. 4; 100 m M NaCl, 0. 10× trypsin 0. The narrow bandwidth used by the model increases measurement accuracy and broad analysis over. PC12 or BV-2 cells treated with various concentrations of BBD were stressed with hypoxia for one hour and the supernatant was then assayed for LDH activity. 306 plate air cal. using SpectraMax i3 (Molecular devices, San Jose, USA). The supernatants were mixed with an LDH assay reagent, which was purchased from Takara Bio Inc. SpectraMax 384 Plus. 2 g/L solution; dilute 1:10 in HBSS. Continuous kinetic monitoring of enzyme activity was performed on SPECTRAmax 340 or Flexstation 2-384 microplate reader (Molecular Devices, Sunnyvale, CA, USA) and controlled by the Softmax software. After treatment, total cellular RNA was extracted with a cold RNA extraction solution (Ultraspec RNA;Call 1-800-745-2710. For use with Spectramax 340 devices which have an 8-pin mini DIN serial socket. Analysis of ALA by Ehrlich’s Reagent Assay. TheSpectraMax®Plus384addstheabilitytoread SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. 7. Products : SpectraMax Plus 384 reader, SpectraMax 190 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax 250 (Obsolete), SpectraMax 340 (Obsolete), VersaMax reader How does stray light affect my OD reading? The basic problem caused by stray light (light you don't want, getting into the read) is a falsely low OD seen. Changes in cell viability were reflected by changes in optical density detected at 450 nm, which was measured using a spectrophotometer microplate reader (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). Centrifuge – Eppendorf 5417C with rotor FA 45–30–11. Absorbance was read at 405 nm on a microplate reader (SPECTRAmax 340 PC). The protein concentration was determined spectrophotometrically at 280 nm in a 1 cm quartz cuvette using a SPECTRAmax-340 UV–vis spectrophotometer. TGFα release was calculated as described in the Results. Cell Culture AO391 (derived from human aorta) and CD314 (derived from human carotid artery) cell lines were a generous gift from Wendy Bauer (Tufts Molecular Cardiology Research Institute) and were grown in DMEM (CellGro) supplemented with 10% FBS. The results of ELISA optical density (OD) values in duplicates were averaged for the following analyses. After the addition of sPLA2 (20 μ g), the reaction mixture was incubated for 40 min at 37°C, and the absorbance was read at 10 min intervals. The values were corrected for nonspecific background by subtracting the OD 650. The optical system of the SpectraMax 340PC384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. ): 8. 5 g/L-EDTA 0. Capabilities include fluorescence and luminescence readings, environmental control, cell viability assays. Other technologies such as imaging. Application note showing how SpectraMax® i3x and SpectraMax®iD5 Multi-Mode Microplate Readers are used to performGq-coupled receptor assays using HTRF technology. Spectramax 340pc User Manual, Hudson River Saranac Pellet Stove Manual, Alesis Midiverb 1 Manual, User Manual For Samsung Galaxy Ace Duos S6802, Di Style Manual, R150f Transmission Repair Manual, Eos5d Mark 2 Manual. The relative cell viability was determined as the percentage of the absorbance value in the cells to the absorbance value of a control group. Wavelength Selection. Perkin Elmer Wallac Victor2 1420 Multilabel Counter $1,500. 1 mg mL −1 CDDP loaded A-CHAM, B-CHAM, C-CHAM or D-CHAM. Products : SpectraMax Paradigm reader, SpectraMax Plus 384 reader, SpectraMax 250 (Obsolete), SpectraMax L reader, SpectraMax 340PC 384 (Obsolete), Gemini EM reader, Gemini XPS reader, SpectraMax 340 (Obsolete). Enzyme activities at high concentration were analyzed using a SpectraMAX 340 plate reader (Molecular Devices) at 380 nm in the kinetic mode at 37°C. 340/80 nm excitation with 616/10 nm emission range Optimized for use with ScanLater Western Blot Detection cartridge for the SpectraMax® i3, i3x, and Paradigm® Multi-Mode Readers Optimized for use with the enhanced TRF detection module for the SpectraMax iD5 Multi-Mode Microplate ReaderThe optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. 5% phosphoric acid) in microplates at room temperature for 10 min. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or. Calculating % of total mediator release in RBL assay. Our absorbance plate readers feature our PathCheck Sensor technology and. SpectraMax 340 Absorbance Microplate Reader, Molecular Devices (CAT#: STEM-SPO-0021-ZJF) Labsystems Fluoroskan II Fluorescent Microplate Reader, Excitation Wavelength Range 320 – 700 nm, Labsystems (CAT#: STEM-SPO-0006-LGZ) BioTek MQX200 Microplate Reader Spectrophotometer, 24VDC, BioTek Instruments, Inc (CAT#: STEM. . density was measured at 570 nm (SpectraMax 340, Molecular De-vices, Sunnyvale, CA). Molecular Devices SpectraMax 340 PC 384 Microplate Reader. With SOFTmax PRO, the contents of the wells in a microplate can be mixed the industry-leading microplate reader software for the SpectraMax Plus 384 system, allows complete customization of data collection and analysis. SpectraMAX 340 plate reader (Molecular Devices). Details. 5 mm (bottom read) Beam diverges to focal spot near bottom of well. All data were normalized to experimental day 0. mums. SpectraMax Multi-Mode Microplate Readers. Briefly, cells were seeded into a. After washing, the dye was eluted with 3% HCl and the absorbance measured at 665 nm with a SpectraMax 340 spectrophotometer (Molecular Devices, Sunnyvale, CA). The Molecular Devices SpectraMAX absorbance microplate readers mimic a dual beam spectrophotometer with a patented multichannel optical design. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. 1 mL of acetylacetone. Streamline GLP/GMP compliance. plates can be used in the SPECTRAmax PLUS 384. Molecular Devices Spectramax 340PC instructions. Gerritsma,B. Alves, Amelia K. 0 nm increments Wavelength Bandwidth: 2 nm Wavelength Accuracy: < ± 1. 1% N-1-naphtylethylenediamine dihydrochloride, 2. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. ac. Molecular Devices Spectramax 340PC instructions. EX range: 340/80 nm EM range: 616/10 nm High fg levels of Streptavidin High fg levels of Streptavidin 2 AlphaScreen. One plate carrier adapter is provided with the instrument. 0. Narrow bandwidths deliver increased measurement accuracy and linearity over a wide range of. All assays were performed in triplicate, and the absorbance at 405 nm was measured using a SpectraMax 340 multiwell plate reader (Molecular Devices). SpectraMAX 340. This is a used Molecular Devices SpectraMax 340PC Microplate Spectrophotometer. MCE has not independently confirmed the accuracy of these methods. SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. The optical system of the SpectraMax 340PC384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). Binding of capture antibodies to polystyrene beads and biotinylation. Read one sample or up to 384. After adding the stop solution, the absorbance at 450 nm with a wavelength correction at 540 nm was measured with a SpectraMax 340 plate reader (Molecular Devices, San Jose, CA, USA). Below is the list of Molecular Devices microplate readers and liquid handling instruments that are of out-of-production and are now considered obsolete. Cet appareil ISM est confomre à la norme NMB-001 du Canada. In this study we used tetanus toxoid (TT) as a well-defined antigen to. #N207) LAL Reagent Water (Lonza cat. 00. Money Saved: $340. The possi-ble effect of zinc or copper on the binding of heme by A was tested as described above by using a 50 and 100 M final concentration of each metal. Operators can conveniently select up to 6 wavelengths at a time for. Sandwich ELISAs were used to measure insulin (DY8056, R&D Systems, Minneapolis, MN, USA). Statistical analysis. The optical density of each sample was analyzed at 450 nm with a reference reading at 630 nm using a SpectraMax 340 absorbance platereader (Molecular Devices, Union City, CA, United States). Wound healing assay. The amount of liberated pNA product during incubations was determined using a standard curve obtained at 410 nm for various concentrations of a pNA stock solution in the SpectraMax 340 microplate reader. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. SoftMax Pro. After then, 10% of ammonium hydroxide solution was added to neutralize the aqueous solution of the ARS extracts, and the absorbance values were measured using a SpectraMax® 340 plate reader at 405 nm. The cell growth was determined by measuring the optical density at 600 nm using a spectrophotometer (SpectraMax 340, Molecular Devices, USA). . g. Like CD3, the CD95 death receptor requires binding of immobilized or multimeric antibodies to be activated effectively. (1993). Developed plates were read on MSD reader Sector Imager S 600, Model 1200, with Discovery Workbench Software (version 4. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. SPECTRAmax 340PC 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. S. D2. Summary of Contents for Molecular Devices SPECTRAmax 340PC. The maximum specific growth rate μ was calculated from the exponential growth phase ( 52 ) and plotted against the concentration of the different drugs. Both readers feature an 8-channel read head for fast reads and. Samples were considered “positive” if the absorbance value was greater than two times the average absorbance value of the negative control wells . Also for: Spectramax 190, Spectramax 340pc384, Versamax. 024). Create custom fluorescence assays without having to purchase expensive filter sets. The cell survival. Lactate dehydrogenase (LDH) release assay Cytotoxicity was determined by measuring the release of LDH. , Sunnyvale, CA, USA) at 595 nm. Molecular Devices SpectraMax Gemini XS Microplate Reader. plates can be used in the SPECTRAmax PLUS 384. The cell concentration was measured by a spectrometer (SpectraMax 340, Molecular Devices, USA) with an optical density at 600 nm (OD 600). 305 reference level saturation. Donor filter: 620 nm. 6, 2 mmol/l DTT, 10 mmol/l MnCl 2 and 10 mmol/l pNPP; the PP2Cα enzyme concentration was 400nmol/l. 1: SpectraMax Plus 384 components. SoftMax Pro. . The Molecular Devices SpectraMax 340PC384 Microplate Reader can run both standard spectrophotometer and microplate reader applications on the same instrument. Microplate Readers. Molecular Devices. The SPECTRAmax 340PC can measure. Multi-mode Microplate Readers. They are for reference only.